The epidemiological burden of reducing salt intake in Paraguay: A modeling study / A carga epidemiológica da redução do sal no Paraguai: um estudo de modelagem

ABSTRACT Objective This study aims to estimate the epidemiological burden of excessive salt intake reduction and achieve the World Health Organization salt reduction target for 2025 in Paraguay, in 2019. Methods We used the Preventable Risk Integrated Model, a comparative risk assessment macro-simulation model, to estimate the averted deaths, disease incidence, and disability-adjusted life years from cardiovascular disease attributable to salt intake in the population of Paraguay for different salt reduction policy scenarios. Results As a result, in Paraguay, excessive salt intake (over 5 g/day) is responsible for approximately 2,656 cardiovascular disease deaths (95% Uncertainty Interval: 1,250-3,765), 4,816 cardiovascular disease cases (95% UI: 2,251-6,947), and 60,529 disability-adjusted life years (95% UI: 27,828-86,258) per year. By reducing salt consumption by 30%, as recommended by the World Health Organization until 2025, approximately 1,188 deaths (95% UI: 520 to 1,820), 2,100 incident cases (95% UI: 923-3,234), and 27,272 disability-adjusted life years (95% UI: 11,999-41,675) from cardiovascular disease could be averted every year. Conclusion In conclusion, the burden of cardiovascular disease attributable to excessive salt intake is significant and salt reduction policies must become a priority in Paraguay.

RESUMO Objetivo Este estudo visa estimar a carga epidemiológica do consumo excessivo de sal e o alcance da meta da Organização Mundial de Saúde para 2025 no Paraguai, em 2019. Métodos Foi usado um modelo de avaliação comparativa de risco (Preventable Risk Integrated Model) para análise comparativa de risco para estimar mortes, casos incidentes e anos de vida ajustados por incapacidade (DALYs) por doenças cardiovasculares atribuíveis ao consumo excessivo de sal na população paraguaia em diferentes cenários. Resultados No Paraguai, o consumo excessivo de sal é responsável por aproximadamente 2.656 mortes (Intervalo de Incerteza 95%: 1.250-3.765), 4.816 casos incidentes (95% II: 2.251-6.947) e 60.529 DALYs (95% II: 27.828-86.258) por doenças cardiovasculares por ano. Com uma redução de 30% no consumo de sal, como recomendado pela Organização Mundial de Saúde até 2025, aproximadamente 1.188 mortes (95% II: 520-1.820), 2.100 casos incidentes (95% II: 923-3.234) e 27.272 DALYs (95% II: 11.999-41.675) por doenças cardiovasculares poderiam ser prevenidos ou adiados por ano. Conclusão Concluiu-se que a carga de doenças cardiovasculares atribuível ao consumo excessivo de sal no Paraguai é significante e políticas de redução deveriam ser priorizadas no país.

Consumo de riesgo para alcohol durante el tiempo de cuarentena por la pandemia del COVID-19 en Paraguay, 2020: encuesta virtual

Introducción: Para la contención de la pandemia causada por la enfermedad del coronavirus (COVID-19) se aplicaron una serie de medidas sanitarias a toda la población, como el distanciamiento social, cierre de fronteras y cuarentena en el hogar. Dichas medidas, además, pudieron haber repercutido en un deterioro de la salud en general, un aumento del consumo de bebidas alcohólicas y otras sustancias para contrarrestar o reducir la intensidad de su respuesta a estas consecuencias. Objetivo: Determinar las características demográficas del consumo de riesgo para alcohol durante el tiempo de cuarentena estricta por la pandemia de covid-19. Metodología: Estudio observacional analítico de corte transverso tipo exploratorio. Constó de 16.538 encuestas virtuales realizadas a personas mayores de edad pertenecientes a todo el territorio nacional entre junio y septiembre del 2020. Se excluyó a 219 encuestados por no cumplir criterios de inclusión o datos incorrectamente llenados. Se determinó el consumo de riesgo mediante una prueba que calificaba el consumo por puntajes. Más de 5 puntos para hombres, más de 4 para mujeres. Resultados: 12224 participantes adultos (18-65 años) con una media de 31,9 años (±9,2), formaron parte del análisis, con predominio de participación masculina 64,5% (n=10531). El 74,9% (n=12224) proveniente del Eje Metropolitano. Según géneros, el OR fue 1,15 IC95 (1,08 - 1,23), vp=0,001. Al comparar nivel educativo, secundaria completa/incompleta, el OR fue 1,56 IC95 (1,19 - 2,06), vp=0,001. Y al comparar por ejes de residencias el OR fue 0,83 IC95 (0,77 - 0,89), vp=0,001. Discusión: Se pudo observar que, durante el tiempo de cuarentena, el consumo de alcohol crecía conforme el nivel educativo de la población aumentaba. Los hombres presentaron un consumo de riesgo mayor que los hombres. Conocer este comportamiento ayudaría a plantear acciones que ayuden a prevenir el consumo de alcohol en el grupo de mayor riesgo.

Introduction: The impact of the outbreak of pandemic proportions of COVID-19 has initiated several health and social measures worldwide. Thus, there is growing concern about the consequences of preventive social confinement associated with the pandemic due to the potential increase in the consumption of alcohol and other harmful substances. Between 2010-2014, in Paraguay approximately 5% of deaths over 15 years of age are attributable to alcohol consumption and are associated with external cause injuries, cancers, liver disease, cardiovascular diseases, addictions and domestic violence. Early detection and treatment are recommended intervention strategies to prevent these consequences. Objective: To determine the demographic characteristics of alcohol risk consumption during the covid-19 pandemic quarantine period. Methods: Analytical cross-sectional, exploratory observational study. It consisted of 16,538 virtual surveys carried out on persons of legal age throughout the national territory between June and September 2020. A total of 219 respondents were excluded for not meeting the inclusion criteria or for incorrectly filled out data. Risk consumption was determined using a test that rated consumption by scores. More than 5 points for men, more than 4 for women. Results: 12224 adult participants (18-65 years old) with a mean age of 31.9 years (±9.2), were part of the analysis, with a predominance of male participation 64.5% (n=10531). The 74.9% (n=12224) came from the Metropolitan Axis. According to gender, the OR was 1.15 CI95 (1.08 - 1.23), pv=0.001. When comparing educational level, high school complete/incomplete, the OR was 1.56 CI95 (1.19 - 2.06), pv=0.001. And when comparing by axes of residence, the OR was 0.83 CI95 (0.77 - 0.89), pv=0.001. Discussion: It was observed that, during the quarantine period, alcohol consumption increased as the educational level of the population increased. Males presented a higher risk consumption than males. Knowing this behavior would help to propose actions to help prevent alcohol consumption in the most at-risk group.

Patrones de actividad física y comportamiento sedentario en adolescentes de 13 a 15 años en Paraguay / Patterns of physical activity and sedentary behaviors in adolescents aged 13 to 15 in Paraguay

Introducción: La actividad física insuficiente es uno de los principales problemas de salud pública a nivel global. Los patrones de conducta en los adolescentes, y el estilo de vida, podrían afectar su salud física y mental. Objetivos: El objetivo de este estudio fue conocer los patrones de actividad física y los comportamientos sedentarios en la población de adolescentes a nivel nacional. Materiales y métodos: Estudio cuantitativo, observacional, descriptivo de corte transverso, se aplicó el cuestionario de la Encuesta Global de Salud Escolar en adolescentes escolares del octavo y noveno grados del 3° ciclo de la Educación Escolar Básica y al 1°, 2° y 3° cursos de la Educación Media de 49 escuelas y colegios del país. En este estudio fueron incluidos 1.803 estudiantes de edades comprendidas entre 13 a 15 años. Resultados: El 27% de los adolescentes de 13 a 15 años de Paraguay son activos, siendo significativamente mayor en hombres que en mujeres (p-valor 0,000) y el 22% son inactivos con mayor frecuencia en mujeres que en hombres (p-valor 0,000). Se observo que el 33,5% de los adolescentes tenían comportamiento sedentario, el 43,4% de los adolescentes no utilizo el desplazamiento activo para asistir a la escuela. Los adolescentes que no participaron de las clases de educación física en la escuela representaron el15,6%. Conclusión: Si bien en un 27% los adolescentes de 13 a 15 años son activos, es preocupante el gran porcentaje de adolescentes inactivos y con comportamiento sedentario.

Introduction: Insufficient physical activity is one of the main public health problems globally. Teen behavior patterns and lifestyle may affect their physical and mental health. Objectives: The objective of this study was to know the patterns of physical activity and sedentary behaviors in the adolescent population nationwide. Materials and methods: A quantitative, observational, descriptive cross-sectional study, the questionnaire of the Global School Health Survey was applied in school adolescents of the eighth and ninth grades of the 3rd cycle of Basic School Education and the 1st, 2nd and 3rd year of Secondary Education in 49 schools and colleges in the country. 1,803 students aged 13 to 15 years were included in this study. Results: 27% of adolescents between the ages of 13 and 15 in Paraguay are active, being significantly higher in men than in women (p-value 0.000) and 22% are inactive more frequently in women than in men (p-value 0.000). It was observed that 33.5% of the adolescents had sedentary behavior, 43.4% of the adolescents did not use active displacement to attend school. Adolescents who did not participate in physical education classes at school accounted for 15.6%. Conclusion: Although 27% of adolescents between the ages of 13 and 15 are active, the large percentage of inactive adolescents with sedentary behavior is worrying.

Características de consumo de tabaco en jóvenes estudiantes en Paraguay / Characteristics of tobacco use in young students in Paraguay

Introducción: Actualmente el tabaquismo es una de las mayores amenazas para la salud pública a nivel mundial. La muerte atribuida al tabaco en Paraguay representa el 12,2% de todas las muertes y el costo de la enfermedad asociada al consumo de tabaco en el sistema de salud es elevado. Objetivos: El objetivo de este estudio es describir las características del consumo de tabaco en jóvenes en Paraguay. Materiales y métodos: Se realizó un estudio descriptivo transversal no probabilístico de muestreo estandarizado con representatividad nacional en estudiantes matriculados del 7° al 9° grado de la educación escolar básica, de 13 a 15 años de ambos sexos que incluyo a 6.518 estudiantes, correspondiente a 25 escuelas de la capital y 25 escuelas en el resto del país. Resultados: La prevalencia actual de consumo de productos de tabaco fue del 7%, fumadores actuales de 3,9% y fumadores de cigarrillos electrónicos de 3,7%. La prevalencia de fumadores de segunda mano fue más del 20% en todos los escenarios estudiados. Alto porcentaje de adolescentes con acceso fácil a la compra de cigarrillos sin verificación de la edad. Más del 50% con acceso a mensajes publicitarios sobre tabaco. Casi un 30% tenían conocimiento del efecto dañino del tabaco y más del 80% se manifiestan favorables a la prohibición de fumar en espacios cerrados y abiertos. Conclusión: Aunque la prevalencia de fumadores en adolescentes no es muy elevada, es un problema de salud pública creciente. Se requiere fortalecer las medidas de prevención y control.

Introduction: Currently, smoking is one of the greatest threats to public health worldwide. Death attributed to tobacco in Paraguay represents 12.2% of all deaths and the cost of the disease associated with tobacco consumption in the health system is high. Objectives: The objective of this study is to describe the characteristics of tobacco consumption in young people in Paraguay. Materials and methods: A non-probabilistic descriptive cross-sectional study of standardized sampling was carried out with national representativeness in students enrolled from 7th to 9th grade of basic school education, from 13 to 15 years old of both sexes that included 6,518 students, corresponding to 25 schools in the capital and 25 schools in the rest of the country. Results: The current prevalence of tobacco product use was 7%, current smokers 3.9%, and electronic cigarette smokers 3.7%. The prevalence of second-hand smokers was more than 20% in all the scenarios studied. High percentage of adolescents with easy access to the purchase of cigarettes without age verification. More than 50% with access to advertising messages about tobacco. Almost 30% were aware of the harmful effect of tobacco and more than 80% are in favor of the prohibition of smoking in closed and open spaces. Conclusion: Although the prevalence of smoking in adolescents is not very high, it is a growing public health problem. It is necessary to strengthen prevention and control measures.

Comportamiento alimentario en adolescentes, en el marco de la encuesta global de salud escolar Paraguay, 2017 / Eating behavior in adolescents, in the framework of the global school health survey

Introducción: Comer saludablemente puede contribuir a disminuir la frecuencia de enfermedades crónicas evitables y a mejorar la calidad de vida en la edad avanzada. El objetivo fue describir el comportamiento alimentario de los adolescentes escolares de escuelas públicas, subvencionadas y privadas a nivel nacional. Materiales y Métodos: Se realizó un estudio descriptivo transversal cuantitativo no probabilístico, como instrumento se utilizó el cuestionario de la Encuesta Global de Salud Escolar, a adolescentes de 12 departamentos, la encuesta fue aplicada en marzo del 2017, fueron incluidos 3149 estudiantes matriculados sistemáticamente con una probabilidad proporcional a los grados Octavo-Tercer curso. Resultados: El comportamiento alimentario se caracterizó por el bajo consumo de frutas y verduras en porciones de cinco o más veces al día que fue solo del 7,5%. El 61,7% consumieron bebidas gaseosas azucaradas una o más veces al día, y 15% de los estudiantes consumieron tres días o más comidas rápidas en los últimos 7 días. Conclusión: El comportamiento alimentario de los adolescentes se caracteriza por un elevado consumo de los alimentos marcadores de una alimentación no saludable con mayor proporción entre los más jóvenes.

Introduction: Eating healthy can help reduce preventable chronic diseases and improve quality of life in old age. The objective was to describe the eating habits of school adolescents from public, subsidized and private schools nationwide. Materials and Methods: It is a non-probabilistic quantitative cross-sectional descriptive study, the instrument used was the Global School Health Survey, for adolescents from 12 departments, the survey was applied in March 2017. 3149 systematically enrolled students were included with a proportional probability to grades Eight-Third year. Results: The eating behavior was characterized by the low consumption of fruits and vegetables in portions of five or more times a day, which was only 7.5%. 61.7% consumed sugary soft drinks one or more times a day, and 15% of the students consumed fast foods three or more days a week. Conclusion: The eating behavior of adolescents is characterized by a high consumption of foods that are markers of an unhealthy diet, with a higher proportion among the youngest.

Actitudes, conocimientos y comportamientos sobre el consumo de sal según la percepción de un grupo de mujeres en Paraguay / Attitudes, knowledge and behaviors about salt consumption according to the perception of a group of women in Paraguay

Introducción: la hipertensión arterial se encuentra dentro de las enfermedades crónicas más prevalentes en nuestro país, el consumo excesivo de sal podría ser un factor preponderante de esta situación, el conocer lo que piensa nuestra población sobre ello y que actitud y predisposición al cambio demuestra, aportaría información relevante para desarrollar estrategias de prevención. Objetivos: El objetivo fue comprender el significado, el valor y el alcance que los sujetos atribuyen al consumo de sal/sodio alimentario y sus actitudes y prácticas para el cambio en relación al consumo de sal/sodio en la alimentación. Materiales y métodos: se enmarcó en un proyecto cualitativo, que permitió indagar sobre los conocimientos y actitudes de las personas sobre el consumo de sal/ sodio alimentario, la adquisición de los productos a consumir y las condiciones para intentar un cambio de actitud y comportamiento. Resultados: fueron agrupados de acuerdo a las categorías y descriptores definidos según el problema de investigación. Conclusión: Esta investigación nos ha llevado a comprender mejor el pensamiento y la actitud hacia el consumo de sal de un grupo población muy importante, que son las mujeres, en quienes recae la tarea de comprar y cocinar para la familia. A través de la información obtenida se crea un abanico de posibilidades para intentar un cambio positivo y reforzar aquello que ya está encaminado.

Introduction: arterial hypertension is one of the most prevalent chronic diseases in our country, excessive salt consumption could be a major factor in this situation, knowing what our population thinks about it and the attitude and predisposition to change it, would provide relevant information to develop prevention strategies. Objectives: The objective was to understand the meaning, value and scope that the subjects attribute to the consumption of salt/sodium in the diet. Materials and methods: it was framed in a qualitative Project, which made it possible to inquire about people's knowledge and attitudes about the consumption of dietary salt/sodium, the acquisition of the products to consume and conditions to attempt a change in attitude and behavior. Results: they were grouped according to the categories and descriptors defined according to the research problem. Conclusion: this research has led us to better understand the thinking and attitude towards salt consumption of a very important population group, that are the woman, in whom falls the task of buying and cooking for the family. Through the information obtained, a range of possibilities is created to try a positive change and reinforce what is already underway.

Nivel de actividad física en la población adulta del Paraguay. Prevalencia y factores asociados / Level of physical activity among adult population in Paraguay. Prevalence and associates factors

Introducción: Los niveles de actividad física determinan las condiciones de salud y enfermedad de las poblaciones. El estudio pretende determinar la prevalencia de los niveles de actividad física (AF) en la población adulta en el Paraguay e identificar los factores asociados a la inactividad física. Métodos: Estudio transversal tipo encuesta poblacional con representatividad nacional, incluyó personas con edades de 15 a 74 años. El muestreo fue probabilístico, trietápico sin reemplazo. STEPSwise fue la metodología y encuesta aplicada durante junio-setiembre 2011. Se consideraron los niveles de actividad física según el cuestionario Mundial sobre Actividad Física. Los valores de AF fueron estimados en MET. Los valores determinados se distribuyeron según las variables sociodemográficas y clínicas. Por regresión logística se estimó asociación entre las variables, en odd ratios con intervalos de confianza del 95%. Resultados: Se incluyeron 2536 participantes. Resultaron inactivos el 27,2% de la población, 20,1% y 31,3% hombres y mujeres, respectivamente. Los grupos que mostraron una mayor prevalencia de inactividad física con diferencias estadísticamente significativas fueron las mujeres, el grupo de edad superior a 55 años, vivir en área urbana, contar con un mayor nivel de estudios, así como un mayor nivel de ingresos. El sobrepeso y la obesidad se asociaron a una mayor prevalencia de inactividad física, principalmente en los varones. Conclusiones: El estudio además de la prevalencia de AF, aporta datos que serán de gran utilidad como línea de referencia para la vigilancia de los factores de riesgos de las enfermedades crónicas no transmisibles. Además contribuye a identificar los subgrupos prioritarios para intervenciones en salud pública.

Introduction: Physical activity levels determine health conditions and diseases in whole populations. The study attempts to determine the prevalence of physical activity (PA) levels in Paraguay and to identify factors associated with physical inactivity. Methods: A cross-sectional population-type survey with national representatively, included people aged between 15 and 74 years old. Sampling was probabilistic, tri-stage without replacement. STEPSwise was the methodology and survey applied during June-September 2011. The levels of physical activity were considered according to the Global Physical Activity Questionnaire (GPAQ). The PA values were estimated in MET. The determined values were distributed according to sociodemographic and clinical variables. Logistic regression was made for estimate the association between the variables in odd ratios with 95% confidence intervals. Results: 2536 participants were included. 27,2% of the population was inactive, 20,1% and 31,3% men and women, respectively. The groups that showed a higher prevalence of PA with statistically significant differences were: women, the age group over 55 years old, living in the urban area, having a higher level of studies and a higher level of income. Overweight and obesity were associated with a higher prevalence of physical inactivity, mainly in males. Conclusions: The study is the first to present the prevalence and differences of levels of global physical activity in Paraguay and related factors. This first step of surveillance of chronic diseases is very useful to provide a baseline for new interventions in public health.

Responsabilidad social universitaria en el curriculum de la carrera de medicina de la FCM-UNA del levantamiento de demandas y necesidades a la declaración del perfil genérico del egresado / University social responsibility in the curriculum career of medicine at the FCM-UNA About the obtainment of demands and needs to the development of a quality assurance system in medical training

Este trabajo se realiza en el contexto de un Proyecto de Innovación Curricular (PIC) de la FCM-UNA, basado en un modelo desarrollado por K, Troncoso y sobre la base de las tendencias y lineamientos actuales de la educación superior y la formación profesional universitaria que orienta y guía la construcción, tanto en el diseño como en la instalación y gestión, de currículos universitarios basados en competencias. Considera Hitos, fases, criterios y productos y forma parte de una propuesta metodológica y conceptual que se ha utilizado como marco de referencia y que permite organizar y estructurar la innovación curricular en la carrera de medicina. El hito 1 se refiere al levantamiento de información de las demandas y necesidades de formación a través del análisis de fuentes de investigación, actores claves y diversos marcos de referencia, para organizar y estructurar compromisos formativos para la elaboración y declaración del perfil de egreso. Por lo tanto significa justificar las decisiones tomadas, argumentando desde la lógica de la formación, la racionalidad que se espera modelar y los problemas, situaciones o contexto sobre los que el egresado actuará, utilizando como referencia las respuestas y posición frente al levantamiento de información. Esto responde a exigencias por parte de la sociedad, y así también para poder instalar una propuesta educativa con formación profesional universitaria pertinente. El hito 2 se refiere al análisis y síntesis de la información obtenida de los actores relevantes, de manera a tomar decisiones sobre cuáles son las demandas y necesidades en la formación de los estudiantes de la FCM-UNA. De esta forma ir avanzando hacia la declaración de los compromisos formativos y su representación en el perfil de egreso.

This work is done in the context of Curricular Innovation Project (CIP) of the FCM UNA, based on a model developed by K, Troncoso and based on current trends and guidelines of superior education and degree training that guides the construction, both in the design and installation of university curricula based on competencies. It considers milestones, stages, criteria and products, and forms part of a methodological and conceptual approach which has been used as a reference that allows to organize and structure the curriculum innovation in medical career. Milestone 1 refers to the obtainment of demands and needs of training through the analysis of research sources, stakeholders and various frameworks, to organize and structure training commitments for the development and declaration of the graduate profile. Therefore it means justify the decisions taken, arguing from the logic of training, the rationality that is expected to model and the problems, situation or context on which the graduate will act, using as reference the responses and position on the collection of information This responds to demands from society, and thus also to install an educational proposal with relevant university training. The milestone 2 refers to the analysis and synthesis of information from relevant stakeholders, in order to make decisions about what are the demands and needs in the training of students of the FCM-UNA. Thus moving towards the declaration of training commitments and its representation in the graduate profile.

Engineering a catabolic pathway in plants for the degradation of 1,2-dichloroethane.

Plants are increasingly being employed to clean up environmental pollutants such as heavy metals; however, a major limitation of phytoremediation is the inability of plants to mineralize most organic pollutants. A key component of organic pollutants is halogenated aliphatic compounds that include 1,2-dichloroethane (1,2-DCA). Although plants lack the enzymatic activity required to metabolize this compound, two bacterial enzymes, haloalkane dehalogenase (DhlA) and haloacid dehalogenase (DhlB) from the bacterium Xanthobacter autotrophicus GJ10, have the ability to dehalogenate a range of halogenated aliphatics, including 1,2-DCA. We have engineered the dhlA and dhlB genes into tobacco (Nicotiana tabacum 'Xanthi') plants and used 1,2-DCA as a model substrate to demonstrate the ability of the transgenic tobacco to remediate a range of halogenated, aliphatic hydrocarbons. DhlA converts 1,2-DCA to 2-chloroethanol, which is then metabolized to the phytotoxic 2-chloroacetaldehyde, then chloroacetic acid, by endogenous plant alcohol dehydrogenase and aldehyde dehydrogenase activities, respectively. Chloroacetic acid is dehalogenated by DhlB to produce the glyoxylate cycle intermediate glycolate. Plants expressing only DhlA produced phytotoxic levels of chlorinated intermediates and died, while plants expressing DhlA together with DhlB thrived at levels of 1,2-DCA that were toxic to DhlA-expressing plants. This represents a significant advance in the development of a low-cost phytoremediation approach toward the clean-up of halogenated organic pollutants from contaminated soil and groundwater.

Apparent lack of pharmacological effect of steviol glycosides used as sweeteners in humans. A pilot study of repeated exposures in some normotensive and hypotensive individuals and in Type 1 and Type 2 diabetics.

Steviol glycosides, isolated from the plant Stevia rebaudiana (Bertoni) Bertoni, have been used as safe sweetening agents for more than 30 years. Beneficial effects of high doses of steviol glycosides on hyperglycemia and hypertension have been previously described when these abnormalities are present. This study was designed to evaluate the effects of steviol glycosides on blood glucose and on blood pressure (BP) in 3 groups of individuals. This was a randomized, double-blind, placebo-controlled, long-term study in three groups of patients: Group 1: subjects with Type 1 diabetes; Group 2: subjects with Type 2 diabetes; and Group 3: subjects without diabetes and with normal/low-normal BP levels. The subjects in each group were randomly allocated to active treatment (the steviol glycoside stevioside: 250mg t.d.s.) or to placebo treatment and followed-up for 3 months. Post-treatment systolic BP, diastolic BP, glucose and glycated hemoglobin (HbA1c) were not significantly different from baseline measurements, except for the placebo Type 1 diabetics group where a significant difference was observed for systolic BP and glucose. No side effects were observed in the two treatment groups. This study shows that oral steviol glycosides, taken as sweetener are well tolerated and have no pharmacological effect.

Progress in understanding the sources, deposition and above-ground fate of trichloroacetic acid.

AIM AND SCOPE: This paper is a companion to the recent review paper by Laturnus et al. (2005) on TCA in soils, presenting a complementary review of knowledge gaps in the sources and fate of trichloroacetic acid (TCA) in plants. MAIN FEATURES: The review considers the various sources of TCA precursors, including the question of how much atmospheric TCA comes from naturally-produced precursors, and addresses the implications of climate change on atmospheric TCA formation. Models of the conversion of precursors to TCA in the atmosphere are critically compared with field measurements of concentrations, deposition and budgets; data on the quantitative relationships between gas-phase TCA, particulate TCA, and TCA dissolved in rain and clouds are reviewed. Methods for quantifying TCA are summarised, along with a description of what the different techniques measure, and how results can be compared. A distinction is made between 'extractable' TCA and 'total' TCA in vegetation. Evidence for the various pathways by which TCA enters plants is given, including the in situ production of TCA in leaves. This leads to a better understanding of how plant tissue concentrations depend on uptake, production and removal rates. Finally, knowledge of the toxic effects of TCA on plants and TCA metabolism in plant tissues is summarised. RESULTS AND DISCUSSION: The discussion highlights knowledge gaps, and is intended to aid the reader in interpreting previously published results through identifying where different ways of expressing data have been used, and the consequent conclusions that can be drawn. CONCLUSION AND FURTHER RESEARCH DIRECTIONS: Recommendations are given for future research directions--in identifying precursor sources, quantifying heterogeneous atmospheric processes, recognising and quantifying uptake pathways, and elucidating the biochemical mechanisms involved in sequestering and degrading TCA inside leaves.